Enzymatic Synthesis of Modified Dna by Pcr and Its Application: a Study on Synthesis and Applications of Modified Dna - Mohammad Mehedi Masud - Livros - LAP LAMBERT Academic Publishing - 9783659188435 - 13 de novembro de 2012
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Enzymatic Synthesis of Modified Dna by Pcr and Its Application: a Study on Synthesis and Applications of Modified Dna

Mohammad Mehedi Masud

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Enzymatic Synthesis of Modified Dna by Pcr and Its Application: a Study on Synthesis and Applications of Modified Dna

Thymidine analogue bearing a methyl ester at the C5 position were synthesized. Thermophilic family B DNA polymerases accepted these analogues as substrate to form the corresponding PCR product. Modified DNA containing this analogue were prepared by PCR with KOD Dash DNA polymerase and were further post-modified to form the corresponding derivatized DNA. These DNA could act as a template for PCR and could accept natural TTP or modified analogue as substrate. The thymidine analogue with an amino-linker was also reacted with fluorescein isothiocyanate, yielding a new analogue which was used as a substrate for PCR to get the corresponding fluorescent labeled DNA. Finally, a novel modified DNA aptamer for sialyllactose that is ubiquitous component of the cell surface responsible for the infections of several viruses was produced by using SELEX method with strong binding ability.

Mídia Livros     Paperback Book   (Livro de capa flexível e brochura)
Lançado 13 de novembro de 2012
ISBN13 9783659188435
Editoras LAP LAMBERT Academic Publishing
Páginas 124
Dimensões 150 × 7 × 226 mm   ·   203 g
Idioma German